Abstract: In order to characterize the interdependence of all the factors directly in the process of bioleaching polymetallic nodules in deep sea, this paper conducted the aerobic and anaerobic leaching experiments of a microbe catalysis MnO₂-FeS₂-H₂SO₄ system. The dynamic model of valuable metals by microbe catalysis leaching under anaerobic condition, that is the shrinking core model, was established using the DLVO theory. In the oxidation-reduction process of polymetallic nodules and pyrites, the Mn-leaching mechanics under anaerobic condition was the circulate transformation between Fe²⁺ and Fe³⁺ by microbe catalysis. The microbes absorbed on the surface of mineral and accelerated the reaction process, the mineral particles got small continuously during the reaction. The further study on the experiments indicates that retract model curves fit the experimental ones nicely, which verified the reliability of the model.