Abstract: In order to overcome the shortcomings of acellular dermal matrix used as tissue engineering scaffold material, such as bad permeability, low degradation speed, and strong immunogenicity, a new type of collagen scaffold was prepared by applying various chemical, biological and physical comprehensive methods to deal with pigskin. The microstructure of the scaffold was observed under photomicroscope and scanning electron microscopy (SEM), and the in vitro degradation time, permeability, tensile-strength, porosity, shrinkage temperature were tested. The experimental results show that its dermal fibroblasts, lipocytes and interstitial substances between collagen fibers have been removed completely, simultaneously collagen fibers have been appropriately loosen, and the original natural three-dimensional meshwork porous structure is maintained; the permeability is about 3 000 g·m^-2·d^-1, which is fit for wound healing; the degradation time is between 25 h and 50 h and can be controlled by adjusting technological conditions; the tensile-strength is between 10.20 MPa and 11.50 MPa which is better; the shrinkage temperature is between 70℃ and 85℃. All of those indicate that the material overcomes the shortcomings of acellular dermal matrix and has high permeability, tensile-strength, and proper degradation speed. So it conforms to tissue engineering scaffold material requirements.