Abstract: Utilizing the manual simulation method in laboratory a self-assemble multilayer was made through anion-cation polyelectrolyte weak solution. The glass substrate equipped with the self-assemble film was put into the simulated body fluid SBF, and under proper conditions the aggradation of hydroxyl apatite was induced on the film. It is shown that the pH value of the SBF solution has much influence on crystallization of hydroxyl apatite. The most compact crystal was observed at pH 7.40 with the self-assembled film and simulated body fluid. The inducing experiments of bio-mineralization indicate that the self-assemble film made from the polyelectrolyte weak solution can be successfully used in the study of bio-mineralization materials.