Biomineralization of three kinds of porous composites for bone tissue engineering scaffolds in simulated body fluid

Three kinds of porous composites based on poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) and bioactive glass (BG), tricalcium phosphate (TCP), hydroxyapatite (HA) respectively were prepared for born tissue engineering scaffolds. The chemical reactions and in vitro degradation of PHBV/ BG, PHBV/TCP and PHBV/HA porous materials under physiological condition were assessed and compared. The changes in surface micromorphology, composition and structure of the composite scaffolds were investigated before and after immersed in simulated body fluid (SBF) solution. It was found that biodegradation occurred for three kinds of scaffolds which led to the weight loss of the scaffolds after immersed in SBF solution. The biomineralized reaction and ion exchange reaction occurred in PHBV/BG and PHBV/ TCP scaffolds in SBF solution, and the mineralized layer formed on the surface of those scaffolds which was analyzed as hydroxyl carbonate apatite (HCA). There was little biomineralized reaction appeared on PHBV/HA scaffolds during immersed in SBF sulotion.